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Prevention of spontaneous mammary adenocarcinoma in HER‐2/neu transgenic mice by foreign DNA
Author(s) -
Sfondrini Lucia,
Besusso Dario,
Rumio Cristiano,
Rodolfo Monica,
Ménard Sylvie,
Balsari Andrea
Publication year - 2002
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fj.02-0383com
Subject(s) - cpg oligodeoxynucleotide , genetically modified mouse , immune system , innate immune system , cancer research , transgene , cpg site , tlr9 , mammary tumor , her2/neu , medicine , biology , adenocarcinoma , cancer , immunotherapy , systemic administration , immunology , gene , in vivo , breast cancer , gene expression , dna methylation , biochemistry , microbiology and biotechnology
Unmethylated CpG‐oligodeoxynucleotides (CpG‐ODNs) are recognized as a ‘danger signal’ and are potent immunostimulators. To test whether tumors might be prevented by maintaining the innate immune system on continuous alert, proto‐ neu transgenic female mice, which develop spontaneous mammary tumors, were systemically treated with CpG‐ODNs at 10‐day intervals. Tumor incidence and number of tumors/mouse were significantly lower in treated mice compared with the control group. Moreover, CpG‐ODN systemic treatment significantly reduced lung metastases induced by intravenous inoculation of N202.1A cells derived from a spontaneous mammary carcinoma. Growth of established tumors was modestly inhibited after CpG‐ODN systemic treatment but strongly on peritumoral application. Our data indicate that systemic repeated injection of CpG‐ODN to maintain the innate immune system on continuous alert prevents the onset of genetically determined tumors and confers tumor protection when the tumor load is low.—Sfondrini, L., Besusso, D., Rumio, C., Rodolfo, M., Ménard, S., Balsari, A. Prevention of spontaneous mammary adenocarcinoma in HER‐2/ neu transgenic mice by foreign DNA. FASEB J . 16, 1749–1754 (2002)