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Crystal structures of leukotriene A 4 hydrolase in complex with captopril and two competitive tight‐binding inhibitors
Author(s) -
Thunnissen Marjolein M.G.M.,
Andersson Björn,
Samuelsson Bengt,
Wong Chihuey,
Haeggström Jesper Z.
Publication year - 2002
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fj.01-1017fje
Subject(s) - chemistry , hydrolase , stereochemistry , epoxide hydrolase , active site , epoxide hydrolase 2 , enzyme , biochemistry , microsome
ABSTRACT Leukotriene (LT) A 4 hydrolase/aminopeptidase is a bifunctional zinc enzyme that catalyzes the final step in the biosynthesis of LTB 4 , a potent chemoattractant and immune modulating lipid mediator. Here, we report a high‐resolution crystal structure of LTA 4 hydrolase in complex with captopril, a classical inhibitor of the zinc peptidase angiotensin‐converting enzyme. Captopril makes few interactions with the protein, but its free thiol group is bound to the zinc, apparently accounting for most of its inhibitory action on LTA 4 hydrolase. In addition, we have determined the structures of LTA 4 hydrolase in complex with two selective tight‐binding inhibitors, a thioamine and a hydroxamic acid. Their common benzyloxyphenyl tail, designed to mimic the carbon backbone of LTA 4 , binds into a narrow hydrophobic cavity in the protein. The free hydroxyl group of the hydroxamic acid makes a suboptimal, monodentate complex with the zinc, and strategies for improved inhibitor design can be deduced from the structure. Taken together, the three crystal structures provide the molecular basis for the divergent pharmacological profiles of LTA 4 hydrolase inhibitors. Moreover, they help define the binding pocket for the fatty acid‐derived epoxide LTA 4 as well as the subsites for a tripeptide substrate, which in turn have important implications for the molecular mechanisms of enzyme catalyses.

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