Premium
Cell migration through defined, synthetic extracellular matrix analogues
Author(s) -
Gobin Andrea S.,
West Jennifer L.
Publication year - 2002
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fj.01-0759fje
Subject(s) - self healing hydrogels , extracellular matrix , cell adhesion , proteolysis , cell migration , chemistry , ligand (biochemistry) , extracellular , peptide , proteolytic enzymes , adhesion , biophysics , fibroblast , receptor , cell , biochemistry , enzyme , polymer chemistry , in vitro , biology , organic chemistry
We have developed synthetic hydrogel extracellular matrix (ECM) analogues that can be used to study mechanisms involved in cell migration, such as receptor‐ligand interactions and proteolysis. The biomimetic hydrogels consist of bioinert polyethylene glycol diacrylate derivatives with proteolytically degradable peptide sequences included in the backbone of the polymer and adhesive peptide sequences grafted to the network. Hydrogels have been developed that degrade as cells secrete proteolytic enzymes. Adhesive peptide sequences grafted to the hydrogel provide ligands that can interact with receptors on the cell surface to mediate adhesion and spreading. In this study, we have characterized the effects of adhesive ligand density on fibroblast migration through collagenase‐degradable and plasmin‐degradable hydrogels and on smooth muscle cell migration through elastase‐degradable hydrogels. In all three cases, we found that cell migration has a biphasic dependence on adhesion ligand concentration, with optimal migration at intermediate ligand levels. Furthermore, both adhesive and proteolytically degradable sequences were required for cell migration to occur. These synthetic ECM analogues may be useful for 3‐D mechanistic studies of many aspects of cell migration