Premium
Von Hippel‐Lindau tumor suppressor and HIF‐1α: new targets of NSAID inhibition of hypoxia‐induced angiogenesis
Author(s) -
Jones Michael K.,
Szabó Imre L.,
Kawanaka Hirofumi,
Husain Syeda S.,
Tarnawski Andrzej S.
Publication year - 2002
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fj.01-0589fje
Subject(s) - angiogenesis , hypoxia (environmental) , hif1a , vascular endothelial growth factor , cancer research , prostaglandin , hypoxia inducible factors , prostaglandin e2 , sulindac , chemistry , endocrinology , pharmacology , medicine , vegf receptors , biochemistry , nonsteroidal , gene , organic chemistry , oxygen
Nonsteroidal anti‐inflammatory drugs (NSAIDs) block prostaglandin synthesis and impair healing of gastrointestinal ulcers and growth of colonic tumors, in part, by inhibiting angiogenesis. The mechanisms of this inhibition are incompletely explained. Here we demonstrate that both nonselective (indomethacin) and COX‐2‐selective (NS‐398) NSAIDs inhibit hypoxia‐induced in vitro angiogenesis in gastric microvascular endothelial cells via coordinated sequential events: 1) increased expression of the von Hippel‐Lindau (VHL) tumor suppressor, which targets proteins for ubiquitination leading to 2) reduced accumulation of hypoxia‐inducible factor‐1α (HIF‐1α) and, as a result, 3) reduced expression of vascular endothelial growth factor (VEGF) and its specific receptor Flt‐1. Because HIF‐1α is the major trigger for hypoxia‐induced activation of the VEGF and Flt‐1 genes, this could explain how NSAIDs inhibit hypoxia‐induced angiogenesis. Exogenous VEGF and, to a lesser extent, exogenous prostaglandins partly reversed the NSAIDs inhibition of hypoxia‐induced angiogenesis. Taken together, these results indicate that NSAIDs inhibit hypoxia‐induced angiogenesis in endothelial cells by inhibiting VEGF and Flt‐1 expression through increased VHL expression and the resulting ubiquitination and degradation of HIF‐1α. This action of NSAIDs has both prostaglandin‐dependent and prostaglandin‐independent components.