Premium
Triiodothyronine‐mediated upregulation of UCP2 and UCP3 mRNA expression in human skeletal muscle without coordinated induction of mitochondrial respiratory chain genes
Author(s) -
Barbe Pierre,
Larrouy Dominique,
Boulanger Catherine,
Chevillotte Emmanuel,
Viguerie Nathalie,
Thalamas Claire,
Oliva Trastoy Manel,
Roques Marina,
Vidal Hubert,
Langin Dominique
Publication year - 2001
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fj.00-0502fje
Subject(s) - ucp3 , uncoupling protein , skeletal muscle , biology , mitochondrial biogenesis , nrf1 , endocrinology , medicine , respiratory chain , triiodothyronine , adipose tissue , brown adipose tissue , mitochondrial respiratory chain , gene expression , mitochondrion , microbiology and biotechnology , gene , biochemistry , hormone
Triiodothyronine (T3) increases mitochondrial respiration and promotes the uncoupling between oxygen consumption and ATP synthesis. T3 effect is mediated partly through transcriptional control of genes encoding mitochondrial proteins. We determined the effect of T3 on mRNA levels of uncoupling proteins (UCP) and proteins involved in the biogenesis of the respiratory chain in human skeletal muscle and on UCP2 mRNA expression in adipose tissue. Ten young, healthy males received 75 to 100 µg of T3 per day for 14 days. The increase in plasma‐free T3 levels was associated with an increase of resting metabolic rate and a decrease of respiratory quotient. In skeletal muscle, treatment with T3 induced a twofold increase of both UCP2 and UCP3 mRNA levels (p < 0.01). The mRNA levels of cytochrome c oxidase subunits 2 and 4, nuclear respiratory factor 1, mitochondrial transcription factor A, and the co‐activator PGC1 did not change during the treatment. In adipose tissue, UCP2 mRNA levels increased threefold. The direct effect of T3 on skeletal muscle and adipose tissue UCP2 and UCP3 mRNA expression was demonstrated in vitro in human primary cultures. Our data show that T3 induces UCP2 and UCP3 mRNA expression in humans. In skeletal muscle, UCP regulation by T3 is not associated with the transcriptional regulation of respiratory chain proteins.