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Measurement of mRNA levels in tumor xenografts with quantitative autoradiography and in situ hybridization
Author(s) -
Wright Paul S.,
CrossDoersen Doreen E.,
Chmielewski Paula A.,
Bush Tammy L.,
Bitonti Alan J.,
Miller Jerry A.
Publication year - 1995
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.9.2.7781931
Subject(s) - in situ hybridization , biology , microbiology and biotechnology , in vivo , messenger rna , in situ , gene expression , estrogen receptor , gene , chemistry , cancer , breast cancer , biochemistry , genetics , organic chemistry
In situ methodologies allow qualitative and semi‐quantitative analysis of spatial gene expression in whole organisms or tissues. We have applied quantitative autoradiography to in situ hybridizations of sections from human breast tumor xenografts to measure mRNA levels for ornithine decarboxylase, estrogen receptor, transforming growth factor alpha, and glyceraldehyde‐3‐phosphate dehydrogenase. Comparisons of control and tamoxifen‐treated animals show significant decreases in MCF‐7 tumor estrogen receptor mRNA levels in the drug‐treated animals. Combining quantitative autoradiography with in situ hybridization allows measurement of absolute rather than relative mRNA levels for genes of interest, and to monitor effector‐induced changes in these mRNAs in vivo.—Wright, P. S., Cross‐Doersen, D. E., Chmielewski, P. A., Bush, T. L., Bitonti, A. J., Miller, J. A. Measurement of mRNA levels in tumor xenografts with quantitative autoradiography and in situ hybridization. FASEB J. 9, 279–283 (1995)