Premium
The purification of membranes by affinity partitioning
Author(s) -
Persson Anders,
Jergil Bengt
Publication year - 1995
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.9.13.7557020
Subject(s) - membrane , polyethylene glycol , chemistry , polymer , ligand (biochemistry) , phase (matter) , partition coefficient , affinity chromatography , conjugated system , chromatography , partition (number theory) , biophysics , combinatorial chemistry , receptor , biochemistry , organic chemistry , biology , mathematics , combinatorics , enzyme
We describe affinity partitioning as a preparative method for membranes and membraneous structures such as organelles, cells, and viruses. Biospecific affinity partitioning is carried out in aqueous polymer two‐phase systems, commonly with polyethylene glycol and dextran as phase polymers, in an environment compatible with membrane structures. Ideally, two‐phase conditions are chosen to partition the bulk of membrane material into one phase, while the affinity ligand, conjugated to the second phase polymer, will selectively pull the membranes to be isolated into this phase. Suitable ligands include lectins, antibodies, and receptor‐specific agents. Because the method has so far been used successfully in rather few instances, all using high ligand receptor densities in target membranes, the discussion focuses on factors to be considered when developing affinity partitioning conditions using new ligands.—Persson, A., Jergil, B. The purification of membranes by affinity partitioning. FASEB J . 9, 1304‐1310(1995)