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Expression and inducibility of vascular adhesion receptors in development
Author(s) -
Heyward Scott A.,
DuboisStringfellow Nathalie,
Rapoport Rebecca,
Bautch Victoria L.
Publication year - 1995
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.9.10.7542216
Subject(s) - biology , microbiology and biotechnology , receptor , cell adhesion molecule , cell adhesion , haematopoiesis , vcam 1 , endothelial stem cell , signal transduction , icam 1 , immunology , stem cell , cell , in vitro , biochemistry
ICAM‐1 and VCAM are cell adhesion receptors expressed on vascular endothelial cells, and their expression is up‐regulated as part of the inflam‐matory response. Tumor‐derived Py‐4‐1 cells were a source of murine endothelial cells, and they showed increased lymphocyte binding when incubated with TNFα. Py‐4‐1 cells stimulated with TNFα or LPS also had elevated levels of ICAM‐l and VCAM RNAs, with maximum levels at 2 h. Developmental expression of adhesion receptors was assayed in murine yolk sacs and in cystic embryoid bodies (CEBs) that differentiate in vitro from murine embryonic stem cells. ICAM‐ l and VCAM RNAs were expressed in unstimulated yolk sacs and CEBs. Expression was inducible in CEBs by exposure to LPS at all developmental stages, beginning at day 6. The time course of RNA induction in CEBs was similar to that of the Py‐4‐l endothelial cell line. ICAM‐l protein expression was localized to vascular and hematopoietic blood islands in the CEBs. These results show that embryonic cells respond to inflammatory mediators by up‐regulating expression of vascular adhesion receptors, and they imply an early ontogeny for the endothelial cell signal transduction pathway necessary for leukocyte re‐cruitment.—Heyward, S. A., Dubois‐Stringfellow, N., Rapoport, R., Bautch, V. L. Expression and inducibility of vascular adhesion receptors in development. FASEB J. 9, 956‐962 (1995)