Premium
Structure of an anti‐idiotypic Fab against feline peritonitis virus‐neutralizing antibody and a comparison with the complexed Fab
Author(s) -
Ban Nenad,
Escobar Carlos,
Hasel Karl W.,
Day John,
Greenwood Aaron,
McPherson Alexander
Publication year - 1995
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.9.1.7821749
Subject(s) - immunoglobulin fab fragments , chemistry , molecular replacement , antibody , conjugate , neutralizing antibody , feline infectious peritonitis , virology , virus , stereochemistry , complementarity determining region , crystallography , crystal structure , immunoglobulin light chain , biology , immunology , covid-19 , medicine , mathematics , mathematical analysis , disease , pathology , infectious disease (medical specialty)
The crystal structure of anti‐idiotopic Fab 409.5.3, made against an E2 specific feline infectious peritonitis virus‐neutralizing antibody 730.1.4, has been determined in its free form, at 2.9 Å resolution by molecular replacement. This antibody, used as an immunogen, elicits the production of anti‐anti‐idiotypic antibodies that in turn neutralize the virus. The structure of the uncomplexed Fab was refined using constrained‐restrained least squares minimization and simulated annealing in combination with conjugate gradient techniques to a crystallographic R of 0.22 based on 16,482 unique reflections between 20.0 and 2.9 Å. The free anti‐idiotypic Fab shows, when compared to its complexed form, a 5° rotation of its variable light with respect to its variable heavy domain and rearrangement of complementarity determining region loops, which permits optimization of the stereocomplementarity between interacting molecules. This finding supports the induced fit hypothesis for antibody antigen interaction.—Ban, N., Escobar, C., Hasel, K. W., Day, J., Greenwood, A., McPherson, A. Structure of an anti‐idiotypic Fab against feline peritonitis virus‐neutralizing antibody and a comparison with the complexed Fab. FASEB J. 9, 107‐114 (1995)