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EPR characterization of molecular targets for NO in mammalian cells and organelles
Author(s) -
Henry Yann,
Lepoivre Michel,
Drapier JeanClaude,
Ducrocq Claire,
Boucher JeanLuc,
Guissani Annie
Publication year - 1993
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.7.12.8397130
Subject(s) - electron paramagnetic resonance , organelle , characterization (materials science) , chemistry , mitochondrion , site directed spin labeling , adduct , biophysics , biochemistry , biology , nanotechnology , nuclear magnetic resonance , materials science , physics , organic chemistry
Nitric oxide is synthesized in mammalian cells from l ‐arginine or from pharmaceutical drugs. It forms paramagnetic complexes with some metalloproteins, inhibiting key enzymes in DNA synthesis, mitochondrial respiration, iron metabolism, etc. This article reviews how electron paramagnetic resonance spectroscopy helps to detect unambiguously such specific molecular targets for NO in mammalian whole cells and organelles. EPR has also been used for the detection of spin adducts of free NO by spin‐trapping methods.—Henry, Y., Lepoivre, M., Drapier, J.‐C., Ducrocq, C., Boucher, J.‐L., Guissani, A. EPR characterization of molecular targets for NO in mammalian cells and organelles. FASEB J. 7: 1124‐1134; 1993.