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DNA adducts in humans as dosimeters of exposure to environmental, occupational, or dietary genotoxins 1
Author(s) -
Schut Herman A. J.,
Shiverick Kathleen T.
Publication year - 1992
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.6.11.1644258
Subject(s) - carcinogen , dna , adduct , dna damage , chemistry , dna adduct , genotoxicity , biochemistry , toxicity , organic chemistry
The quantitation of adducts of genotoxins with DNA is probably one of the best indicators of genetic damage due to exposure to toxins or carcinogens. It is generally believed that such adducts can lead to mutations, which in turn can trigger the initiation of the carcinogenic process. DNA adducts have been quantitated in white blood cells and in various tissues of smokers, persons in certain high‐exposure occupations, and persons consuming foods contaminated with certain carcinogens. The feasibility of this approach for biochemical epidemiologic studies has been demonstrated using methods such as 32 P‐postlabeling, enzyme‐linked immunosorbent assay, and synchronous fluorescence spectrophotometry. Relatively large interindividual differences in DNA adducts have been observed in both exposed and nonexposed persons. As a result, there are only a few studies in which clear quantitative and qualitative differences between these two groups have been observed. In addition, it appears that in some studies the 32 P‐postlabeling method does not detect the presence of the polycyclic aromatic hydrocarbon DNA adducts that are detectable by immunoassays. More extensive studies in additional populations at risk should shed further light on the utility of DNA adduct analysis in biochemical monitoring, especially if further refinements in methodology would result in increased sensitivity and specificity.— Schut, H. A. J.; Shiverick, K. T. DNA adducts in humans as dosimeters of exposure to environmental, occupational, or dietary genotoxins. FASEB J. 6: 2942‐2951; 1992.