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Inhibition of calcium mobilization is an early event in opiate‐induced immunosuppression
Author(s) -
Sei Yoshitatsu,
McIntyre Todd,
Fride Ester,
Yoshimoto Kohji,
Skolnick Phil,
Arora Prince K.
Publication year - 1991
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.5.8.2022315
Subject(s) - splenocyte , morphine , opiate , immunosuppression , glucocorticoid , cd8 , chemistry , endocrinology , antiglucocorticoid , medicine , pharmacology , calcium , immune system , spleen , immunology , glucocorticoid receptor , receptor
Morphine administered as a subcutaneous implant inhibits the initial increase in cytoplasmic free‐calcium [Ca 2+ ] i induced by mitogens in mouse splenocytes. This effect was not reproduced by incubation of splenocytes with morphine (10 –8 ‐10 –4 M). Analysis of splenocyte subpopulations demonstrates that this effect was manifest in both B and T cells. However, within T cell subpopulations, CD4 + but not CD8 + cells were affected. Adrenalectomy abolished this effect of morphine in CD4 + T but not CD4 − , CD8 − spleen cells (most likely Thy 1.2 − B cells). Moreover, simultaneous administration of the opiate antagonist naltrexone blocked the effect of morphine in CD4 − , CD8 − spleen cells, but not in CD4 + T cells. These data indicate that the effects of morphine on mitogen‐stimulated increase in [Ca 2+ ] i may be mediated through distinct glucocorticoid‐dependent and ‐independent mechanisms. The morphine‐induced inhibition of an increase in [Ca 2+ ]; in immune cells reported here may be an early event mediating opiate‐induced immunosuppression.—Sei, Y.; McIntyre, T.; Fride, E.; Yoshimoto, K.; Skolnick, P.; Arora, P. K. Inhibition of calcium mobilization is an early event in opiate‐induced immunosuppression. FASEB J. 5: 2194–2199; 1991.