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Human cellular protein patterns and their link to genome DNA sequence data: usefulness of two‐dimensional gel electrophoresis and microsequencing
Author(s) -
Celis Julio E.,
Rasmussen Hanne H.,
Leffers Henrik,
Madsen Peder,
Honoré Bent,
Gesser Borbala,
Dejgaard Kurt,
Vandekerckhove Joël
Publication year - 1991
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.5.8.1827083
Subject(s) - biology , human genome , genome , gel electrophoresis , dna , genetics , protein sequencing , gene , sequence (biology) , dna sequencing , sequence analysis , computational biology , homology (biology) , microbiology and biotechnology , peptide sequence
Analysis of cellular protein patterns by computer‐aided 2‐dimensional gel electrophoresis together with recent advances in protein sequence analysis have made possible the establishment of comprehensive 2‐dimensional gel protein databases that may link protein and DNA information and that offer a global approach to the study of the cell. Using the integrated approach offered by 2‐dimensional gel protein databases it is now possible to reveal phenotype specific protein (or proteins), to microsequence them, to search for homology with previously identified proteins, to clone the cDNAs, to assign partial protein sequence to genes for which the full DNA sequence and the chromosome location is known, and to study the regulatory properties and function of groups of proteins that are coordinately expressed in a given biological process. Human 2‐dimensional gel protein databases are becoming increasingly important in view of the concerted effort to map and sequence the entire genome.—Celis, J. E.; Rasmussen, H. H.; Leffers, H.; Madsen, P.; Honoré, B.; Gesser, B.; Dejgaard, K.; Vandekerckhove, J. Human cellular protein patterns and their link to genome DNA sequence data: usefulness of two‐dimensional gel electrophoresis and microsequencing. FASEB J. 5: 2200–2208; 1991.