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Fructose administration stimulates glucose phosphorylation in the livers of anesthetized rats
Author(s) -
Van Schaftingen Emile,
Davies Dewi R.
Publication year - 1991
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.5.3.2001793
Subject(s) - fructose , phosphorylation , administration (probate law) , chemistry , endocrinology , medicine , pharmacology , biochemistry , political science , law
A method allowing one to measure the rate of glucose phosphorylation in the livers of anesthetized rats is described. Upon injection of [2‐ 3 H]glucose into the portal vein, about 90% of the radioactivity remained in the liver for approximately 30 s. The proportion of radioactivity accounted for by tritiated water increased linearly at a rate of about 5%/min in control animals. Fructose injected into the penile vein stimulated this rate up to 2.2‐ and 2.7‐fold in fed and overnight starved rats respectively, a maximal effect being observed at a dose of 50 mg/kg under both conditions. Fructose was also active when administered by intragastric infusion. The ketose caused increases in the concentration of fructose 1‐phosphate, which reached values known to relieve the inhibition exerted on glucokinase by its regulatory protein.—–Van Schaftingen, E.; Davies, D. R. Fructose administration stimulates glucose phosphorylation in the livers of anesthetized rats. FASEB J. 5: 326–330; 1991.