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Protection by acidotic pH against anoxic cell killing in perfused rat liver: evidence for a pH paradox
Author(s) -
Currin Robert T.,
Gores Gregory J.,
Thurman Ronald G.,
Lemasters John J.
Publication year - 1991
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.5.2.2004664
Subject(s) - anoxic waters , lactate dehydrogenase , chemistry , ischemia , reperfusion injury , hypoxia (environmental) , pharmacology , biochemistry , andrology , medicine , endocrinology , oxygen , biology , enzyme , organic chemistry , environmental chemistry
Reperfusion of ischemic tissues causes a paradoxical injury. Here, we measured lactate dehydrogenase (LDH) release as an indicator of tissue damage in perfused rat livers during anoxia and reoxygenation. During anoxia, LDH release was substantially reduced at acidotic pH (pH 6.1–6.9). Using anoxia at pH 6.1 followed by reoxygenation at pH 7.3 to model ischemia and reperfusion, an abrupt release of LDH occurred after reperfusion. A similar release of LDH occurred when pH of anoxic livers was increased to 7.3 without reoxygenation, but LDH release did not occur after reoxygenation at pH 6.1. Thus, a rapid increase of pH rather than reoxygenation accounted for tissue injury after reperfusion of ischemic liver.—Currin, R. T.; Gores, G. J.; Thurman, R. G.; Lemasters, J. J. Protection by acidotic pH against anoxic cell killing in perfused rat liver: evidence for a pH paradox. FASEB J. 5: 207–210; 1991.