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New techniques for physical mapping of the human genome
Author(s) -
Billings Paul R.,
Smith Cassandra L.,
Cantor Charles R.
Publication year - 1991
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.5.1.1846833
Subject(s) - human genome , computational biology , genome , physical mapping , biology , dna , dna sequencing , polymerase chain reaction , genetics , computer science , gene , gene mapping , chromosome
We describe improvements in techniques and strategies used for making maps of the human genome. The methods currently used are changing and evolving rapidly. Today's techniques can produce ordered arrays of DNA fragments and overlapping sets of DNA clones covering extensive genomic regions, but they are relatively slow and tedious. Methods under development will speed the process considerably. New developments include a range of applications of the polymerase chain reaction, enhanced procedures for high resolution in situ hybridization, and improved methods for generating, manipulating, and cloning large DNA fragments. More detailed genetic and physical maps will be useful for finding genes, including those associated with human diseases, long before the complete DNA sequence of the human genome is available.—Billings, P. R., Smith, C. L., and Cantor, C. R. New techniques for physical mapping of the human genome. FASEB J. 5 : 28–34; 1991.