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Transplantation of syngeneic transfected cells to probe the in vivo immune response to viral proteins
Author(s) -
Nakayama Hiroyuki,
Shibata Motohiro,
Wohlenberg Charles,
Rooney James F.,
Notkins Abner Louis
Publication year - 1991
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.5.1.1846831
Subject(s) - transfection , immune system , in vivo , microbiology and biotechnology , transplantation , biology , herpes simplex virus , lymphocyte , antibody , cell culture , immunology , virology , virus , medicine , genetics , surgery
BALB/3T3 cells were transfected with the glycoprotein D (gD) gene of herpes simplex virus (HSV) and a cell line expressing gD on the cell surface was isolated. In vitro, 51 Cr release tests showed that the transfected cells were destroyed by anti‐HSV antibody in the presence of complement. To investigate in vivo immune response, the gD‐transfected cells were transplanted into the footpads of syngeneic HSV‐immunized or unimmunized BALB/c mice. In unimmunized mice, transfected cells remained intact for 7 days or longer, and the site of injection showed only slight lymphocyte infiltration. In contrast, in immunized mice, transfected cells elicited massive lymphocyte infiltration and were mostly destroyed by day 4. Analysis of infiltrating cells revealed that they were mainly Thy1 + and CD8 + lymphoyctes along with small numbers of CD5 + , CD4 + , and B lymphocytes. These studies show that transfected murine cells expressing gD can be used to study the in vivo immune response to single viral proteins and they argue that the immune response contributes to the pathogenesis of HSV infection.—Nakayama, H.; Shibata, M.; Wohlenberg, C.; Rooney, J. F.; Notkins, A. L. Transplantation of syngeneic transfected cells to probe the in vivo immune response to viral proteins. FASEB J. 5: 104–108; 1991.