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Efficient production of chicken egg yolk antibodies against a conserved mammalian protein
Author(s) -
Gassmann Max,
Thömmes Pia,
Weiser Thomas,
Hübscher Ulrich
Publication year - 1990
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.4.8.1970792
Subject(s) - polyclonal antibodies , yolk , antibody , immunization , biology , antigen , immune system , specific antibody , microbiology and biotechnology , immunology , food science
The egg yolk of immunized chicken is a rich and inexpensive source of specific polyclonal antibodies. In this paper we show that 20‐30 μg of a highly conserved mammalian protein, as exemplified by proliferating cell nuclear antigen, are sufficient to induce an immune response. Immunoblot analysis revealed that specific antibodies appeared 20 days after immunization, reached a plateau after 30 days, and remained high until at least day 81. A total amount of 4 g immunoglobulin was extracted from 62 eggs of one immunized hen, yielding approximately 130 mg of specific antibodies.— G assmann , M.; T hömmes , P.; W eiser , T.; H übscher , U. Efficient production of chicken egg yolk antibodies against a conserved mammalian protein. FASEB J. 4: 2528‐2532; 1990.