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Hep G2 cells as a resource for metabolic studies: lipoprotein, cholesterol, and bile acids
Author(s) -
Javitt Norman B.
Publication year - 1990
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.4.2.2153592
Subject(s) - very low density lipoprotein , chylomicron , intermediate density lipoprotein , hep g2 , cholesterol , triglyceride , lipoprotein , reverse cholesterol transport , chemistry , biochemistry , metabolism , apolipoprotein e , medicine , biology , endocrinology , in vitro , disease
Hep G2, a liver cell line derived from a human hepatoblastoma that is free of known hepatotropic viral agents, has been found to express a wide variety of liver‐specific metabolic functions. Among these functions are those related to cholesterol and triglyceride metabolism. Confluent Hep G2 monolayers express normal low‐density lipoprotein (LDL) receptors and continue to internalize and metabolize chylomicrons, very low‐density lipoproteins (VLDL), LDL, and high‐density lipoproteins. In lipoprotein‐free medium, apolipoproteins A‐I, A‐II, B, C, and E accumulate in the medium together with cholesterol, cholesteryl ester, triglyceride, and all the primary bile acids. The regulation of their synthesis and secretion is not fully known and their interrelationships have not been established. Because Hep G2 cells express these and other components of cholesterol and triglyceride metabolism, they are a microcosm for studying the central role of the liver.—J avitt , N. B. Hep G2 cells as a resource for metabolic studies: lipoprotein, cholesterol, and bile acids. FASEB J. 4: 161‐168; 1990.