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Neutrophils contribute to ischemia/reperfusion injury in rat liver in vivo
Author(s) -
Jaeschke Hartmut,
Farhood Anwar,
Smith C. Wayne
Publication year - 1990
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.4.15.2253850
Subject(s) - in vivo , ischemia , reperfusion injury , liver injury , medicine , pharmacology , cardiology , biology , microbiology and biotechnology
To determine the role of neutrophils in the pathogenesis of hepatic ischemia/reperfusion injury, livers from male Fischer rats were subjected to 45 min of no‐flow ischemia followed by reperfusion for up to 24 h. Two phases of liver injury were identified, an initial phase during the first hour of reperfusion and a later progression phase with 80 ± 3% hepatocyte necrosis and an 80‐fold increase of neutrophil infiltration in the liver after 24 h. Pretreatment with a monoclonal antibody against neutrophils, which caused consistent neutropenia, protected the liver from reperfusion injury as indicated by 28 ± 10% necrosis, and 84% reduction of hepatic neutrophil accumulation and a complete recovery of the hepatic ATP content. Our data suggest that the later progression phase of reperfusion injury after hepatic no‐flow ischemia is mediated mainly by neutrophils.—J aeschke , H.; F arhood , A.; S mith , C. W. Neutrophils contribute to ischemia/reperfusion injury in rat liver in vivo. FASEB J. 4: 3355–3359; 1990.