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DNA modification in vivo by derivatives of glucose: enhancement by glutathione depletion
Author(s) -
Shires T. K.,
Tresnak J.,
Kaminsky M.,
Herzog S. L.,
TrucPham B.
Publication year - 1990
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.4.15.2253848
Subject(s) - in vivo , glutathione , chemistry , dna , biochemistry , biophysics , microbiology and biotechnology , biology , genetics , enzyme
When BHK or HTC cells are cultured for 20 min with [U‐ 14 C]glucose in the presence of agents that deplete reduced glutathione, DNA banded from the cells in cesium salt gradients containing guanidium HCl is radioactively labeled. This depletion‐dependent labeling required live cells. It was not caused by reactive contaminants in the radioactive glucose preparations, by carbohydrate or protein comigration into the DNA band, or by metabolism of glucose into deoxyribose. Labeling levels are similar whether depletion is achieved by oxidation (with the drug diamide) or by inhibition of synthesis (with methionine sulfoximine). A temporal association between GSH repletion and the appearance of D‐lactate, the putative unique product of GSH‐dependent glyoxylase action on pyruvaldehyde, suggests possible involvement of 3‐carbon dicarbonyls.—S hires , T. K., T resnak , J., K aminsky , M., H erzog , S. L., and T ruc ‐P ham , B. DNA modification in vivo by derivatives of glucose: enhancement by glutathione depletion. FASEB J. 4: 3340–3346; 1990.