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Establishment and characterization of a clonal line of parathyroid endothelial cells
Author(s) -
Brandi Maria Luisa,
Ornberg Richard L.,
Sakaguchi Kazushige,
Curcio Francesco,
Fattorossi Andrea,
Lelkes Peter I.,
Matsui Toshimitsu,
Zimering Mark,
Aurbach Gerald D.
Publication year - 1990
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.4.13.1698682
Subject(s) - parathyroid hormone , doubling time , endothelial stem cell , parathyroid chief cell , cell culture , medicine , growth factor , endocrinology , vascular endothelial growth factor , biology , chemistry , microbiology and biotechnology , calcium , in vitro , biochemistry , vegf receptors , genetics , receptor
We have isolated endothelial cells derived from bovine parathyroid tissue. These cells have been cloned and maintained by serial passage for more than 40 months without showing signs of senescence. Prolonged culture was accomplished by using a medium favoring endothelial cell growth and methods for enriching endothelial cells in primary culture. The cloned parathyroid endothelial cells contained factor VIII‐related antigen, took up acetylated low‐density lipoproteins and parathyroid hormone, and showed morphological features comparable to other endothelial cells. Bovine parathyroid endothelial cells replicated with a mean doubling time of 65 h. Fibroblast growth factors, platelet‐derived growth factor, and calcium acted as mitogens for parathyroid endothelial cells, whereas transforming growth factor β inhibited proliferation.— B randi , M. L.; O rnberg , R. L.; S akaguchi , K.; C urcio , F.; F attorossi , A.; L elkes , P. I.; M atsui , T.; Z imering , M.; A urbach , G. D. Establishment and characterization of a clonal line of parathyroid endothelial cells. FASEB J. 4: 3152‐3158; 1990.