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Cytochalasin B induces cellular DNA fragmentation
Author(s) -
Kolber Michael A.,
Broschat Kay O.,
LandaGonzalez Belkis
Publication year - 1990
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.4.12.2394319
Subject(s) - fragmentation (computing) , cytochalasin b , dna fragmentation , microbiology and biotechnology , microfilament , dna , cytochalasin d , actin , dna damage , cytoskeleton , chemistry , biology , biochemistry , cell , programmed cell death , apoptosis , ecology
Cellular DNA fragmentation can be induced in many biological instances without plasma membrane damage. The fungal metabolite, cytochalasin B, is capable of modifying numerous cellular functions related to DNA synthesis. In this work it is demonstrated that cytochalasin B is capable of inducing DNA fragmentation in a number of cells lines. This DNA fragmentation occurs before plasma membrane lysis and over a period of hours. Cytochalasin E and villin, agents that act on the microfilaments, also induce DNA fragmentation. Phorbol dibutyrate, a diacylglyceral analog, is able to inhibit cytochalasin B‐induced DNA fragmentation in a dose‐dependent fashion. These findings support the interpretation that cytochalasin B is inducing DNA fragmentation via its effect on the actin filaments.— K olber , M. A., B roschat , K. O., L anda ‐G onzalez , B. Cytochalasin B induces cellular DNA fragmentation. FASEB J. 4: 3021‐3027; 1990.