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The effects of acetate on enhancing the proliferation and preadipocyte differentiation of stromal vascular cells isolated from Hanwoo beef cattle
Author(s) -
Baatar Delgerzul,
Wandita Theresia Galuh,
Cruz Joseph Flores,
Hwang Seong Gu
Publication year - 2017
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.31.1_supplement.lb813
Subject(s) - hanwoo , cellular differentiation , chemistry , oil red o , myogenesis , stromal cell , fatty acid synthase , endocrinology , medicine , beef cattle , biology , fatty acid , adipose tissue , biochemistry , adipogenesis , food science , myocyte , zoology , gene
We investigated the effect of acetate on the proliferation and differentiation of bovine stromal‐vascular cells (SVC). SVC was isolated from the longissimus muscle collected directly from approximately 30‐month‐old Hanwoo beef cattle steers. After confluence, the differentiation was induced by 1‐methyl‐3‐isobutyl‐xanthine, dexamethasone, insulin, and rosiglitaszone for 2 days, and then subsequently cultured for 14 days. The cells were treated with different concentrations of acetate (0.125 to 2 mMol) throughout the differentiation period. Cell viability assay revealed a dose‐dependent increase in the proliferation of SVC. Monitoring of the differentiation through Oil Red‐O staining showed that treatment with acetate up to 1mMol significantly increased cell differentiation and fat accumulation. Supplementation of acetate in the media throughout the differentiation period also enhanced the induction of all differentiation markers. Acetate treatment unregulated the mRNA and protein expression of peroxisome proliferator‐activated receptor gamma (PPARγ), CCAAT/enhancer binding protein alpha (C/EBPα), Sterol regulatory element‐binding protein 1c (SREBP‐1c), fatty acid binding protein 4 (FABP 4) and fatty acid synthase (FAS). These data suggests that acetate can enhance adipocyte differentiation from SVC isolated from Hanwoo beef cattle and possibly improve meat palatability through increased intramuscular fat deposition and reduce meat toughness through decreased fibrogenic differentiation. Support or Funding Information This work was carried out by the support of the Rural Development Administration Grant (Project No. PJ01268703).