Premium
Effects of amaranth ( Amaranthus esculantus ) and canola ( Brassica napus ) oil supplementation on oxidative metabolism of neutrophils in patients with obesity
Author(s) -
Dominika Kanikowska,
Alina Kanikowska,
Rafał Rutkowski,
Małgorzata Włochal,
Marian Grzymisławski,
Maki Sato,
Andrzej Bręborowicz,
Janusz Witowski
Publication year - 2017
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.31.1_supplement.lb715
Subject(s) - canola , oxidative stress , amaranth , reactive oxygen species , brassica , antioxidant , food science , respiratory burst , chemistry , biology , medicine , biochemistry , botany
Reactive oxygen species are postulated to be involved in systemic inflammation and oxidative stress in patients with obesity. Activated polymorphonuclear neutrophils (PMNs) generate high amounts of reactive oxygen species. The aim of the study is to investigate whether amaranth oil (Amaranthus esculantus) and canola ( Brassica napus) oil supplementation impacts on oxidative metabolism in patients with obesity. The hypothesis is that, owing to its lipid‐lowering and antioxidant properties, amaranth oil and canola oil will protect against oxidative stress. In the study, the effects of amaranth and canola oils on oxidative metabolism and apoptosis of neutrophils from obese individuals has been investigated. We tested 19 obese patients (aged 48.3±16 years,6 women and 13 men, BMI = 41.1 ±7.8 kg/m2, (mean ±SD)). The study adhered to basic Principles of the Declaration of Helsinki. Neutrophils were isolated and oxidant production, in response to phorbol 12‐myristate 13‐acetate, was characterized by using luminol dependent chemiluminescence (CL) and flow cytometric dichlorofluorescin oxidation assay. Neutrophils from obese individuals before treatment had a significantly higher H2O2 production and CL response compared to obese after 3 weeks of both oils supplementation (p < 0.05). This amaranth oil and canola oil exert beneficial effects in cells and may, therefore, be useful in the treatment of obesity disorders. Support or Funding Information none