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Bisphenol‐A Alters Human Neutrophil Function
Author(s) -
Balistrieri Angela,
Insel Paul A,
Corriden Ross
Publication year - 2017
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.31.1_supplement.lb696
Subject(s) - chemotaxis , innate immune system , reactive oxygen species , chemistry , calcium , chelerythrine , microbiology and biotechnology , immune system , extracellular , protein kinase c , biochemistry , pharmacology , immunology , biology , signal transduction , receptor , organic chemistry
Bisphenol‐A (BPA), a synthetic organic compound, is frequently present in plastic‐lined cans, water bottles, toys, and teeth sutures. Previous studies have shown that BPA can produce adverse health effects that include defects in reproductive function and altered prenatal/childhood development. However, little is known regarding the effects of BPA on immune function. In this study, we assessed the effect of BPA on human polymorphonuclear neutrophils (PMNs), a critical component of the innate immune system's defense against pathogens. Using the fluorescent reactive oxygen species (ROS) dye DCFDH, we found that BPA induces a concentration‐dependent increase in ROS generation by PMNs. The membrane‐permeable calcium chelator BAPTA‐AM and/or removal of extracellular calcium inhibited BPA‐induced ROS production, indicating that the process is calcium dependent. Pre‐incubation of PMNs with chelerythrine chloride, a protein kinase C (PKC) antagonist, also inhibited BPA‐induced ROS production, implying that this action of BPA is PKC‐dependent. Transwell chemotaxis assays revealed that BPA exposure reduces the chemotactic capacity of PMNs in a gradient of the bacterial cell wall component f‐Met‐Leu‐Phe, a potent chemoattractant. Our findings reveal that BPA alters the in vitro function of PMNs, including ROS production and chemotaxis, and raise the possibility of altered innate immunity in vivo, especially in those with compromised immune function and who can be exposed to BPA in a wide variety of products.