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Endothelial dysfunction occurs independently of adipose tissue inflammation and insulin resistance in ovariectomized Yucatan swine
Author(s) -
Jurrissen Thomas Jacob,
Olver T. Dylan,
Lin Gabriela S,
Winn Nathan C,
Gastecki Michelle L,
Welly Rebecca J,
Grunewald Zachary I,
Emter Craig A,
VieiraPotter Victoria J,
Padilla Jaume
Publication year - 2017
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.31.1_supplement.lb666
Subject(s) - medicine , endocrinology , insulin resistance , ovariectomized rat , inflammation , adipose tissue , white adipose tissue , leptin , endothelial dysfunction , insulin , hormone , obesity
Ovarian hormone deficiency is associated with increased adiposity, inflammation, and impaired vascular function, which are contributing factors to cardiovascular disease. In rodents, ovariectomy causes white adipose tissue (AT) inflammation and insulin resistance (IR), potentially contributing to vascular dysfunction; however, whether this occurs in a more translationally‐relevant large animal model remains unknown. We tested the hypothesis that ovariectomy would promote white AT inflammation, IR and peripheral vascular dysfunction in female swine. At sexual maturity (7 months of age), female Yucatan swine were either ovariectomized (OVX, n=7) or kept ovary‐intact (CON, n=9). All pigs were fed equal amounts of standard chow, and were euthanized 6 months post‐surgery. Body mass, spontaneous (cage) physical activity, fasting blood glucose, insulin, and non‐esterified fatty acids were not significantly different between groups; however, triglycerides were elevated in OVX vs CON (29%, P <0.05). Circulating cytokines (eg. IL‐1β, IL‐6, IL‐10, and TNFα) were not significantly different between groups, whereas, circulating leptin levels were decreased in OVX vs Con (−21%, P <0.05). Adipocyte size in omental (visceral) AT was greater in OVX vs CON (27%; P <0.05); however, no differences in omental AT inflammation were noted between groups. In contrast, ex vivo brachial artery endothelial‐dependent vasorelaxation was reduced in OVX vs CON (−30%, P <0.05) and rescued with blockade of endothelin receptor A via BQ123. In conclusion, loss of ovarian hormones perturbs endothelial function independent of systemic and visceral AT inflammation or IR in Yucatan swine.