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Young healthy men and women are protected from prolonged sitting and postprandial hyperglycemia‐induced brachial artery endothelial dysfunction
Author(s) -
Walsh Lauren K,
Restaino Robert M,
Jones Chelsea A,
Winn Nathan C,
Padilla Jaume
Publication year - 2017
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.31.1_supplement.lb656
Subject(s) - postprandial , medicine , brachial artery , endothelial dysfunction , crossover study , sitting , cardiology , glycemic , endocrinology , insulin , blood pressure , alternative medicine , pathology , placebo
Previous studies have shown that postprandial hyperglycemia and prolonged sitting are predictors of cardiovascular disease (CVD), potentially due to the detrimental effects of glycemic excursions on the artery wall and reductions in shear stress, respectively. It is possible that prolonged sitting, when combined with consumption of high sugar drinks, may lead to severe endothelial dysfunction. Herein, we hypothesized that sitting exacerbates postprandial hyperglycemia‐induced endothelial dysfunction. In twelve young healthy subjects (six men, six women), brachial artery flow‐mediated dilation (FMD) was assessed at baseline, 1 hour, and 2 hours post oral administration of a 75g glucose load (oral glucose tolerance test, OGTT). Subjects were tested on two separate visits in a randomized, crossover design: after either accumulating 15,000 steps or remaining seated, each throughout an eight‐hour period. The average number of steps during the walking and sitting conditions were 15,972 ± 189 and 315 ± 37 (p<0.0001), respectively. FMD was not reduced during hyperglycemia in either the walking (baseline: 5.98± 0.8%, 1hr: 4.99± 1.01%, 2hr: 6.40 ± 1.12%) or sitting (baseline: 5.24 ± 0.8%, 1hr: 4.84± 0.65%, 2hr: 5.97± 0.94%) condition. However, plasma glucose levels were elevated in the final hour of the OGTT in the walking, but not sitting, condition (p<0.01). Additionally, non‐esterified fatty acid (NEFA) levels were also elevated in response to hyperglycemia in the walking condition, supporting the tenet that prior walking promotes a substrate shift towards increased lipid utilization. These preliminary results suggest that young, healthy subjects may be protected against acute hyperglycemia–induced endothelial dysfunction. This effect is associated with increased plasma glucose and NEFAs following the glucose drink, potentially suggesting a difference in substrate utilization between conditions. Support or Funding Information NIH R21DK105368 (JP)