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Effect of Hydrogen Sulfide‐Releasing Compounds on Aqueous Humor Outflow Facility Under Different Perfusion Pressures
Author(s) -
Robinson Jenaye,
Mitchell Leah,
Ngele Kalu,
Opere Catherine,
Ohia Sunny E,
NjieMbye Ya Fatou
Publication year - 2017
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.31.1_supplement.lb606
Subject(s) - perfusion , saline , chemistry , outflow , cysteine , hydrogen sulfide , aqueous solution , basal (medicine) , anesthesia , endocrinology , medicine , biochemistry , enzyme , organic chemistry , sulfur , physics , meteorology , insulin
Hydrogen sulfide (H 2 S)‐releasing compounds have been reported lower intraocular pressure in rabbits and increase aqueous humor outflow facility in a porcine ocular anterior segment model, ex vivo . The aim of the present study was to investigate the pharmacological actions of L‐cysteine, a substrate for the biosynthesis of H 2 S, on aqueous humor outflow facility using the porcine ocular anterior segment model maintained under normal and elevated ocular perfusion pressure. Methods Porcine ocular anterior segment explants were perfused with Dulbecco's Modified Eagle's Medium maintained at 37°C and gassed with 5% CO 2 and 95% under a constant normal perfusion pressure of 7.35 mmHg or under an elevated perfusion pressure of 15 mmHg. Stabilized explants were exposed to L‐cysteine (1 nM – 1 μM) and outflow facility was monitored for 4 hours. Control experiments containing vehicle (0.1% saline) were run in parallel. Results Under normal pressure conditions, L‐cysteine elicited a concentration‐dependent increase in aqueous humor outflow facility that reached a maximal effect at 100 nM [(150.6 ± 17.2% of basal; ( p < 0.04) ], an effect that lasted for a duration of four hours. Interestingly, under conditions of elevated perfusion pressure, L‐cysteine (100 nM) also significantly increased outflow facility reaching a maximal effect of 173.0 ± 23.0% of basal ( p < 0.01) . Conclusion We conclude that L‐cysteine can increase aqueous humor outflow facility in the porcine anterior segment model in a manner that was independent of perfusion pressure. However, further studies are needed to determine the role of H 2 S in regulating aqueous humor outflow under these conditions.