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Antidiabetogenic Effects of the Seed Extracts of Picralima nitida
Author(s) -
Ojuola Oluwatosin Moradeke,
Abatan Matthew Oluwole
Publication year - 2017
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.31.1_supplement.lb562
Subject(s) - glibenclamide , phytochemical , chemistry , ethyl acetate , alloxan , saponin , blood sugar , traditional medicine , sugar , triglyceride , diabetes mellitus , ethanol , glycoside , solvent , blood lipids , chloroform , pharmacology , chromatography , cholesterol , food science , biochemistry , medicine , endocrinology , stereochemistry , alternative medicine , pathology
Picralima nitida is a plant with medicinal importance including its use for lowering blood sugar levels. The extracts of the seed have been evaluated for possible blood sugar lowering properties using alloxan‐induced diabetes mellitus model in rats. The seed material of P . nitida was extracted using gradient solvent extraction with chloroform, diethyl ether, ethyl acetate and methanol. Pilot toxicity studies using a crude extract indicated that the plant was safe when administered orally at 3000mg/kg. The different solvent extracts were administered at a dose of 750mg/kg, to alloxan induced diabetic rats and their blood sugar levels monitored hourly for four hours post administration. Glibenclamide served as the standard antidiabetic drug. Results show that the different solvent extracts had significant blood lowering effects (P<0.5) compared to the untreated diabetic rats. The extracts also produced decrease in the triglyceride, total cholesterol, low density lipoprotein values and an increase in high density lipoprotein levels. The chloroform solvent extract produced the highest bioactivity and was therefore subjected to further tests. The extract was subjected to vacuum liquid chromatography (VLC) and the biograded fractions evaluated for their blood sugar lowering effects. The results showed that fraction 5 (30% ethyl acetate and 70% ethanol) produced the highest blood sugar lowering effects. Standard procedures were used to carry out phytochemical analysis which showed that the plant has alkaloids, flavonoids, saponin, tannins and cardiac glycosides. The evaluation substantiates the medicinal use of P . nitida for lowering blood sugar in diabetic patients Support or Funding Information Pan African University