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3D Human Platelet Lysate Gel Model: LPS and Poly (I:C) Increase Invasion of Human Umbilical Cord Mesenchymal Stem Cells
Author(s) -
Jirakittisonthon Thitikan,
Jimenez Jake,
Abello Javier,
Cromer Adrienne,
Sun Susan,
Weiss Mark
Publication year - 2017
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.31.1_supplement.lb518
Subject(s) - platelet lysate , mesenchymal stem cell , wound healing , umbilical cord , cell migration , stimulation , in vitro , chemistry , microbiology and biotechnology , immunology , biology , biochemistry , neuroscience
Mesenchymal Stromal Cells (MSCs) can promote wound healing and have ability to migrate into injured tissues. The in vitro methods commonly used to track the migration of cells are the scratch assay and the transwell migration assay. Here, the objectives are to develop a novel would model in 3 dimensions and to observe invasion responses of MSCs after stimulation by LPS or Poly(I:C). A 3D wound model was created by culturing MSCs suspended with in a platelet lysate gel. Next MSC growth and migration was evaluated by stimulation by LPS or Poly(I:C). Both LPS‐stimulated, a gram negative bacterial cell wall component, and Poly (I:C)‐stimulation, a representative of viral infection, produced more MSCs growth and invasion than control (3D gel alone). This study demonstrates that MSCs can grow and migrate within a novel 3D wound model and that MSCs respond to LPS and Poly I:C with enhanced growth and migration. This data suggests that MSCs respond with more rapid wound healing response following exposure to stimuli associated with infection.