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The Changes of the Dopaminergic D1 Cell after Stimulation by STN‐DBS in Parkinson's Disease Rats
Author(s) -
Duan Hubin,
Hao Chunyan,
Li Hao,
Wang Suping,
Zhang Ce,
Fan Yimin,
Liu Yueting,
Pei Mingyang,
Wang Huan
Publication year - 2017
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.31.1_supplement.lb512
Subject(s) - stimulation , deep brain stimulation , dopaminergic , subthalamic nucleus , parkinson's disease , dopamine , prefrontal cortex , psychology , neuroscience , medicine , endocrinology , disease , cognition
Objective To investigate the changes of the dopaminergic D1 cell after stimulated by subthalamic nucleus deep brain stimulation (STN‐DBS) in Parkinson's Disease (PD) rats. Methods 55 healthy male SD rats were randomly divided into normal control group (n=15)、 PD group (n=15)、 sham stimulation group (n=15) and stimulation group (n=15). The apomorphine rotation was observed as the symbol of successful PD model. Stimulation and sham stimulation groups were planted in unilateral STN‐DBS. STN‐DBS in stimulation group was powered on and was stimulated consecutive days at the regular time, while Sham stimulation group complied with the same program except power on the STN‐DBS. The latency to find the platform, swimming speed, swimming distance and crossing times were detected with Morris water maze. Immunohistochemistry was used to observe the change and count the dopaminergic D1 receptor cells in hippocampal C1 area and prefrontal cortex. Results After stimulated by STN‐DBS regularly for 7 days, the symptoms of PD rats were improved significantly after stimulated by STN‐DBS. Compared with PD model group and sham stimulation model group, after trained, not only the escape latency of rats in the DBS stimulated model group was decreased (P < 0.01), but also swimming distance (P < 0.05)in the plate quadrant was increased. The dopaminergic D1 receptor cell of hippocampal C1 area and prefrontal cortex in stimulation group increased by compare with that in PD model group and sham stimulation group, furthermore, which there were statistical significance. Conclusion STN‐DBS stimulation not only can improve the ability of PD rats' cognition, but also can protect dopaminergic receptor cells in some degree. Support or Funding Information This study was supported by the National Natural Science Foundation Youth Fund (Fund No. 30600637), China Postdoctral Foundation(Fund No.2014M561207), the Shanxi Province Basic Research Program Youth Science and Technology Research Fund (Fund No. 2010021034‐4), the Shanxi Scholarship Council of China (Fund No. 2011‐096 and Key Projects No.4), The science and technology research projects of health department of Shanxi Province(Fund No.2016D011119).