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Implication of Aberrant Protein Expression in the Etiology of Pancreatic Cancer
Author(s) -
Nyaga Simon G,
Akobundu Blessing,
GiwaOtusajo Jamiu,
Hazel Kaisha
Publication year - 2017
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.31.1_supplement.lb499
Subject(s) - pancreatic cancer , biology , cancer research , cell growth , metastasis , cancer , cell culture , microbiology and biotechnology , biochemistry , genetics
Pancreatic cancer is a leading cause of cancer mortality in the U.S. American Cancer Society estimates that there will be 53,670 new cases and 43,090 pancreatic cancer death in 2017 in the U.S. Although many risk factors are known, the precise mechanisms involved in the development/progression of the malignant phenotype of this devastating disease are poorly understood. The interaction of the risk factors with cellular DNA may result in abnormal protein changes that could affect the functions of essential biological pathways in cells. The goal of this study is to understand the molecular mechanisms involved in the etiology of pancreatic cancer. To address our goal, we focused on identification of differentially expressed proteins in a pancreatic cancer cell line, BxPC‐3, relative to a nonmalignant pancreatic cell line, HPDE‐6. The cell lines were cultured and cell‐free extracts prepared followed by protein separation by SDS polyacrylamide gel electrophoresis (SDS‐PAGE) and staining using Coomassie blue. Specific candidate proteins were identified by liquid chromatography in tandem with mass spectrometry (LC‐MS/MS). Several proteins were shown to be differentially expressed including; MYH10, CEACAM6, and IGFBP‐5 which were confirmed to be highly over‐expressed (approximately 5×, 10× and 5× respectively) in BxPC‐3 cell line compared to HPDE‐6 cell line using Western Blot analysis. Interestingly, these proteins are associated with biological processes such as cell proliferation, differentiation, apoptosis, cell adhesion, and metastasis: processes commonly altered in many cancers. This aberrant protein expression could be associated with abnormal protein functions and interactions that may be critical in the initiation/progression of pancreatic cancer. Hence, these findings implicate IGFBP‐5, CEACAM6, and MYH10 in the etiology of pancreatic cancer. Further biochemical characterization may yield biomarkers useful in effective therapeutic intervention of pancreatic cancer. Support or Funding Information Department of Biology, Morgan State University