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Protective Effects of Mucuna Seed Extract against Oxidative and Inflammatory Stress in Murine Microglia and Human Neuroblastoma Cells
Author(s) -
Ma Hang,
Johnson Shelby L.,
DaSilva Nicholas A.,
Liu Yongqiang,
Yuan Tao,
Seeram Navindra P.
Publication year - 2017
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.31.1_supplement.lb412
Subject(s) - mucuna pruriens , neuroprotection , pharmacology , mucuna , nitric oxide , oxidative stress , reactive oxygen species , microglia , viability assay , chemistry , inflammation , antioxidant , lipopolysaccharide , traditional medicine , apoptosis , biochemistry , medicine , immunology , organic chemistry , psychiatry
Mucuna ( Mucuna pruriens ; commonly known as velvet bean) is a medicinal plant that has been traditionally used in Ayurvedic medicine for various conditions including diabetes, inflammation, and brain dysfunctions. It contains levodopa (L‐DOPA) and is used for the treatment for Parkinson's disease (PD). Although the anti‐PD effects of Mucuna have been associated with its L‐DOPA content, recent studies suggest that other bioactive compounds may also contribute to its protective effects against PD. Herein, we initiated this study to develop and evaluate a Mucuna pruriens extract (MPE) for the neuroprotective effects using in vitro assays. The MPE (at 60 μg/mL) scavenged 73.7 % of free radicals in the DPPH assay. The MPE was evaluated for anti‐neuroinflammatory effects in murine microglia BV‐2 cells. The MPE (at 50 and 25 μg/mL) significantly reduced lipopolysaccharide (LPS) induced nitric oxide production by BV‐2 cells by 60.1 and 37.8%, respectively, as compared to cells treated with LPS alone. In addition, the MPE (at 50 and 25 μg/mL) decreased the production of H 2 O 2 ‐induced reactive oxygen species by 79.9 and 64.5%, respectively. Lastly, the MPE was evaluated for neuroprotective effects in human neuroblastoma SH‐SY5Y cells. MPE treatment (at 1 μg/mL and 10 ng/mL) significantly reduced 6‐hydroxydopamine‐induced cytotoxicity and increased cell viability by 73.1 and 75.1%, respectively. In summary, this study supports the neuroprotective effects of MPE and warrants further investigation of its potential as a dietary management for neurodegenerative diseases, in particular, PD. Our future studies will include the identification of the bioactive constituents in MPE responsible for its anti‐PD effects.