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Effect of Excess Iron Intake on Growth and Intestinal Inflammatory Injury in Rats
Author(s) -
Ma Yan,
Sun Yongye,
Jiang Yicheng,
Ma Aiguo
Publication year - 2017
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.31.1_supplement.lb411
Subject(s) - chemistry , dietary iron , ferritin , medicine , small intestine , iron status , iron group , histopathology , zoology , body weight , normal group , iron deficiency , endocrinology , biochemistry , biology , anemia , pathology , organic chemistry , metal
Objective To study the effects of excess iron intake on growth and development, and determine the possible mechanism. Methods Fourty eight male Wistar rats were randomly divided into 4 groups: low iron group, normal group, medium iron group, and high iron group, and received daily iron intake of 7mg/kg, 16.8mg/kg, 35mg/kg, and 70mg/kg in diet, and deionized water, respectively, for 8 weeks. The general condition, body weight, and food intake were documented during the study. Serum levels of Ferritin, IL‐6, IL‐10, and sIgA were measured using enzyme‐linked immunosorbent assay(ELISA). Results During the process of iron intervention, the high iron group had lower growth compared with the other three groups ( p <0.05). In addition, the levels of IL‐6 (108.60pg/ml) in small intestine were higher in the high iron group compared to the normal iron group (84.68pg/ml, P <0.05), while serum levels of IL‐10 (50.47 pg/ml) in small intestine in the high iron group was lower than that in the normal iron group (71.69 pg/ml, ( P <0.05). Serum levels of sIgA (62.3pg/ml) in small intestine with the high iron group is lower than that in the normal iron group (67.1 pg/ml, P <0.05). The histopathology showed normal morphology in the low iron and normal iron groups, however the rats in medium iron group showed slightly damaged mucosa, and lost apical microvilli. Irregular shape of microvilli and necrosis were found in a few epithelial cells of intestinal mucosa in the high iron group rats. Conclusion Excessive dietary iron intake has a negative affect on the normal growth and development of rats, and results in inflammatory injury in intestinal tract of rats. Support or Funding Information The National Natural Science Fund (No. 81373000)