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Gupan extracts increased the expression level of genes related to growth hormone
Author(s) -
Kang MyungHwa,
Moon HaeSung
Publication year - 2017
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.31.1_supplement.lb298
Subject(s) - myogenesis , medicine , endocrinology , gene expression , anterior pituitary , skeletal muscle , growth factor , hormone , bovine somatotropin , biology , growth hormone , rna , chemistry , gene , receptor , biochemistry
Growth hormone (GH) is the main regulator of longitudinal growth before puberty, and treatment with recombinant human GH (rec‐hGH) can increase muscle strength. Most physiological actions of GH are mediated by changes in gene transcription. GH is a 191‐amino acid pituitary protein that stimulates the hepatic production and release of insulin‐lilke growth factor‐1 (IGF‐1) into the systemic circulation. GH deficiency may occur as an isolated disorder or as part of multiple hormone deficiencies. The standard treatment of GH deficiency in adults and children is replacement with rec‐hGH. Current rec‐hGH therapy requires daily subcutaneous injections that may decrease compliance. But, these treatment evoked side effects, such as injection site reactions, edema, and arthralgia. In this study, we tested the exterio part of back (termed: Gupan) extracted from Geoclemys reevesii Gray. C2C12 murine skeletal muscle cells were cultured in DMEM supplemented with 10% FBS, 25 mM Hepes until they reached 80% confluence. Then, differentiation medium was added DMEM (5% horse serum), and the cells were allowed to differentiate into myotubes during 4 days. After 16 h in serum‐free medium, C2C12 myotubes were stimulated with Gupan (15 ug/ml) and rec‐hGH (500 ng/ml) for 6 h. RNA was obtained from each sample using Total RNA purification kit and cDNA was prepared form 250 ng of RNA using the high‐capacity cDNA reverse transcription kit. qRT‐PCR was conducted with FastStart Essential DNA Green Master (Roche). qRT‐PCT was used for confirmation of gene expression changes induced by rec‐hGH. Nine genes were confirmed as being differentially expressed after rec‐hGH induction. Among 9 genes, 3 genes (CISH, npy1r,ugdh) were highly expressed more than rec‐hGH. 2 genes (BTC, Bcl6) were also highly expressed more than no treatment. The other 2 genes (SOCS2, tgfbr3) was a little increased in the expression. However, BMP4 and RGS2 were not increased. Although we do not test many experiments, this study shows modifications in the gene expression profile of C2C12 cell line treated with Gupan in vitro. Further investigations need to be study with potential implications in the action of GH.