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EGCG, An Active Ingredient in Green Tea, Modulates Cell Proliferation in Human Pancreatic Cancer Cells and Rat Osteosarcoma Cells in vitro
Author(s) -
Lai Nok Hang Tony,
Bautista Josue Kevin,
Rodriguez Abigail,
Bolivar Samuel,
Joseph Eugene E
Publication year - 2017
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.31.1_supplement.lb29
Subject(s) - cancer cell , cell growth , pancreatic cancer , cancer research , apoptosis , osteosarcoma , cancer , cell culture , angiogenesis , cell , biology , chemistry , medicine , biochemistry , genetics
Cancer is characterized by the uncontrolled proliferation of abnormal cells. Traditional treatment of cancer include surgery, radiation, and chemotherapy. Many studies have been done to elucidate the response of cancer cells to a variety of treatments. However, not many studies have been conducted on how cancer cells respond to natural compounds that are purported to have therapeutic effects on cancer cells. (‐)‐Epigallocatechin‐3‐gallate (EGCG) is a powerful ingredient of green tea which has been shown to induce apoptosis in tumor cells, prevent angiogenesis and modulate the invasion and migration of cancer cells. Pancreatic and bone cancer cells are among some of the most malignant forms of cancer. In this study, it was hypothesized that EGCG inhibits cell proliferation of both human pancreatic (Panc‐1) and rat osteosarcoma (UMR 106‐01 BSP) cell cultures, inhibits the expression of CXCR‐4, 14‐3‐3σ, and VEGFA proteins in pancreatic cancer cell cultures, and regulates mineralization in UMR 106‐01 rat sarcoma cell cultures. Panc‐1 and UMR106‐01 cell cultures were routinely passaged, treated with different concentrations of EGCG (0, 20, 40, 60, 80 and 100μM) in 6 well plates, then incubated for 48 hours at 37 degrees Celsius under humidification and 5% carbon dioxide infusion. At the end of the experiment, cells were counted using cell flow cytometry. Designated Panc‐1 cell cultures were treated with different concentrations of EGCG. After routine electrophoresis of control and experimental samples, Western blotting technique was employed to identify the expression of Panc‐1 proteins CXCR‐4, 14‐3‐3σ, and VEGFA. F test ANOVA was used to compare variances and all values in the results were expressed as means ±SD. The cell proliferation results from the Panc‐1 and UMR 106‐01 cell lines showed a decreasing trend in cell proliferation in a concentration‐dependent manner. At lower concentrations there was an increase in proliferation whereas at higher concentrations (>80uM) there was a decrease in proliferation. The results of the Western blotting technique showed that the expression of Panc‐1 proteins CXCR‐4, 14‐3‐3σ, and VEGFA are inhibited in a concentration‐dependent manner when treated with EGCG. The proliferation results suggest a dual effect of EGCG on cell proliferation both in Panc‐1 and UMR 106‐01 cancer cell cultures. EGCG may provide a therapeutic effect on both cancer cells lines at higher concentrations. The results from the Western blotting suggest that EGCG exerts its inhibitory effects on Panc‐1 cell proliferation through the regulation of the expression of the Panc‐1 proteins CXCR‐4, 14‐3‐3σ, and VEGFA. Support or Funding Information This research was supported by the innovations fund of the Biology Department of La Sierra University in Riverside, CA.