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Validation of a Macronutrient Mixed Challenge Beverage for Personalized Nutrition Applications using a Reduced Sampling Period
Author(s) -
Winters Barbara L,
Anthony Joshua C,
Remensperger Allison,
Boorsma Andre,
Vandenbroek Tim,
Maki Kevin
Publication year - 2017
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.31.1_supplement.lb289
Subject(s) - bloating , medicine , flatulence , calorie , population , triglyceride , nausea , crossover study , food science , cholesterol , environmental health , chemistry , alternative medicine , pathology , placebo
Oral carbohydrate, lipid and protein tolerance tests have been used to track changes in physiology, inform health status, and provide impetus for dietary changes. Tests typically take 4–8 hours, and require multiple blood draws, limiting the ability to use challenge testing outside of clinical settings for consumer wellness applications. The objective of this study was to validate the use of a mixed macronutrient challenge beverage using a truncated time course and reduced sampling protocol as part of a personalized nutrition and wellness program. Participants (n=18) were recruited from the general population for an IRB approved, double‐blind randomized study. Following on overnight fast, blood was collected via finger pricks and applied to dried blood spot cards, they then consumed the macronutrient mixed challenge beverage (20 g protein, 75 g carbohydrate and ≥50 % calories from fat (≥12 g fat/100 g) within 5 minutes of beverage consumption (t = 0 min) and finger prick blood samples were collected at 30, 60, 90, 120, 180 and 240 min. The mean ± SEM fasting triglyceride (TG) concentration was 112 ± 13.6 mg/dL, with a robust increase of 46 ± 12.7 mg/dL to 159 ±19.6 mg/dL at 120 min post beverage consumption, p=0.002. A gastrointestinal questionnaire was collected pre‐consumption and at 240 min. Overall the beverage was well tolerated and there were no significant increases from pre‐consumption in the percentage of subjects reporting somewhat more or much more than usual nausea, abdominal pain, flatulence, bloating or diarrhea. However, 14% of participants reported somewhat or much more than usual gastrointestinal rumblings (p<0.05) during the 240‐min period after beverage consumption. Collectively, these data show that the challenge beverage can be used to assess TG response at 120 min, and showed the feasibility of finger pricks for dried blood spot collection. These results support the view that challenge testing can be scaled for use in consumer wellness programs. Support or Funding Information Habit, LLC