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Consumption of Bifidobacterium animalis subsp. lactis BB‐12 reduces TNF‐α secretion from LPS‐stimulated PBMCs, but does not alter serum TNF‐α levels
Author(s) -
VanEvery Hannah Leigh,
Meng Huicui,
Flemming Jennifer A,
Lee Yujin,
Ba Zhaoyong,
Furumoto Emily J,
Roberts Robert F,
KrisEtherton Penny M,
Rogers Connie J
Publication year - 2017
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.31.1_supplement.lb287
Subject(s) - bifidobacterium animalis , probiotic , peripheral blood mononuclear cell , tumor necrosis factor alpha , immune system , bifidobacterium , immunology , cytokine , secretion , lactobacillus , medicine , microbiology and biotechnology , in vitro , biology , fermentation , bacteria , food science , biochemistry , genetics
Increasing evidence suggests that probiotic bacteria can modulate inflammatory responses. However the immune response varies by species and strain of organism, as well as delivery matrix of the probiotic. Furthermore the tissue compartment and assay method by which inflammatory mediators are quantified in clinical trials is a debated issue. The goal of the current study was to evaluate the effect of one probiotic species, Bifidobacterium animalis subsp. lactis BB‐12 at a dose of log 10 ± 0.5 CFUs/day on both plasma‐ and in vitro cultured peripheral blood mononuclear cells (PBMC)‐derived inflammatory mediators in a partially blinded, 4‐period crossover, free‐living study. Healthy adults (n=30) aged 18–40 years were recruited, and received 4 treatments in a random order: 1) yogurt smoothie alone; smoothie with organism added 2) before or 3) after fermentation, or 4) organism given in capsule form. At baseline and after each treatment, plasma was collected and PBMCs were isolated and stimulated in vitro with LPS as an inflammatory stimulus. Inflammatory cytokines were quantified in each compartment at baseline and after each treatment. Participants who consumed yogurt smoothies with BB‐12 added post‐ fermentation had a significant reduction in TNF‐α secretion from LPS‐stimulated PBMCs compared to baseline (p=0.039). However, there was no treatment effect observed in plasma TNF‐α levels. Additionally, plasma TNF‐α levels were not correlated with TNF‐α produced by cultured PBMCs in the presence of stimulation. These results demonstrate that quantification of inflammatory mediators in the plasma of healthy adults may not be adequate to detect the effect of probiotic consumption on inflammatory responses. The assessment of a nutritional intervention on in vitro cultured PBMCs in response to LPS stimulation may be a more sensitive assay method to detect probiotic‐induced changes in inflammatory responses. These findings demonstrate that selection of the tissue compartment and the assay method to evaluate inflammatory response is a critical variable in clinical trial design. Support or Funding Information Dairy Research Institute