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Prophylactic Effect of Lotte Probiotic Chocolat © Administration on Loperamide ‐ induced Constipation in Rats
Author(s) -
Lee ChulSang,
Tan PeiLei,
Yoon SeokMin,
Choi DaHye,
Kim SaiHun
Publication year - 2017
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.31.1_supplement.lb281
Subject(s) - constipation , probiotic , medicine , loperamide , lactobacillus plantarum , lactobacillus , streptococcus thermophilus , bloating , gastroenterology , diarrhea , food science , biology , bacteria , lactic acid , genetics , fermentation
Constipation is defined as a stressful defecation which can lead to the risks of bowel obstruction and life‐threatening complications. Probiotics have received increasing attention as a natural way of promoting health and preventing diseases. Members of the genera Lactobacillus and Bifidobacterium, in particular; have been shown to promote gastrointestinal health through modulation of gut microbiota and gut immunity. Owing to their health‐promoting benefits, probiotic bacteria could be used for the treatment of constipation. It has also been suggested that chocolate can be used as a potential cryoprotectant candidate and carrier for delivering a probiotic formulation targeted to the colon. In previous studies, we found that consumption of Lotte Probiotic Chocolat © containing Streptococcus thermophilus MG510 and L.plantarum LRCC5193 improved symptoms of constipation in loperamide ‐ induced constipation rat model. However, very little work has addressed the potential protective effect of probiotic chocolate against constipation. Therefore, this animal trial was designed to investigate the potential prophylactic effect of Lotte Probiotic Chocolat © on loperamide – induced constipation in rats. Sixty Sprague Dawley rats were divided into six groups; N (no constipation), C (constipation), CHO (constipation + chocolate), Lotte Probiotic Chocolat © (constipation + chocolate + 10 10 CFU/day of Streptococcus thermophilus MG510 and Lactobacillus plantarum LRCC5193), BB12 (constipation + chocolate + 10 10 CFU/day of B. animalis subsp. lactis BB12), BL6 (constipation + chocolate + 10 10 CFU/day of L. brevis subsp. coagulans ). Feed and water was fed in ad libitum . The rats were treated for a total of 4 weeks and constipation was induced by daily loperamide (5mg/kg) administration at the fourth week. At the end of experiment, colon and blood serum were collected for biochemical and histological analyses. Additionally, fecal samples were also collected once a week during the pre‐treatment period, and three times in the constipation induction period for microbial profile analysis. Our results showed that MG510 + LRCC5193‐fed group had significantly (P< 0.05) higher mRNA expression levels of tight junction proteins (occludin, claudin, ZO‐1), thicker colonic mucosa and a higher number of goblet cells compared to the N and C groups. Moreover, MG510 + LRCC5193 group also had significantly (P< 0.05) higher motility score compared to the C and CHO groups. Fecal analyses also further revealed that administration of MG510 + LRCC5193 is not only increased and normalized fecal moisture content, but also altered the composition of gut microbiota. Hence, these results collectively demonstrate that 4 week administration of Lotte Probiotic Chocolat © can modulate the gut microbiota and improve intestinal functions in ways that could attenuate the risk of constipation.