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Biological roles of oligosaccharides on recombinant eel LH and functions of eelLH receptors
Author(s) -
Byambaragchaa Munkhzaya,
Kang MyungHwa,
Kim DaeJung,
Min KwanSik
Publication year - 2017
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.31.1_supplement.lb246
Subject(s) - glycosylation , japanese eel , luteinizing hormone , receptor , recombinant dna , biology , pngase f , mutant , hormone , blot , n linked glycosylation , follicle stimulating hormone , secretion , protein subunit , biochemistry , microbiology and biotechnology , glycoprotein , japonica , gene , botany , glycan
The goandotropins (GTHs) follicle‐stimulating hormone (GTH1) and luteinizing hormone (GTH11) are produced in the pituitary gland of all vertebrates, including fish species, and they are key regulators of gonadal development and reproduction. In this study, we produced the deglycosylated mutants of rec‐eelGTHll and analyzed their biological activity using eel LH receptor cell lines. In the hormone secretion, the glycosylation site as Asnα 56 dosen't affect, while glycosylation sites at Asnα 79 and Asnβ 10 have a little effect at the hormone expression into the medium. In western blotting analysis, rec‐LH was detected bands of 32 kDa and the molecular weight was decreased about 2–3 kDa in the single glycosylation site mutants. Deglycosylation treatment by PNGase F was decreased to 25 kDa at the molecular weight of all mutants. Oligosaccharides at Asn 56 on α subunit and at Asn 10 on β subunit are crucial for eelLH biological activity by using cell lines expressing eel LH receptor. Support or Funding Information This work was supported by the project “Seeding production of eel, Anguilla japonica by artificial induction of sexual maturation” (R2017003).