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The optical spectroscopic properties of glycation induced changes in hemoglobins
Author(s) -
Sriramoju Vidyasagar,
Dudley Raymond,
Secor Jeffrey A,
Ashrafi Solyman,
Alfano Robert R
Publication year - 2017
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.31.1_supplement.lb223
Subject(s) - glycation , hemoglobin , fluorescence , fluorophore , chemistry , glycosylation , chromophore , raman spectroscopy , biophysics , absorption (acoustics) , glycated hemoglobin , photochemistry , biochemistry , diabetes mellitus , materials science , medicine , endocrinology , type 2 diabetes , biology , optics , physics , receptor , composite material
The glycation induced changes in human hemoglobin is studied using absorption, fluorescence, Raman and time resolved fluorescence spectroscopic methods. The non‐enzymatic glycosylation of hemoglobin as a result of sustained elevated blood sugar levels changes the chemistry of its native chromophore and fluorophore moieties as a result it's tertiary structure gets altered significantly. These post translational molecular modifications would induce changes in optical properties like absorption, fluorescence emissions, fluorescence lifetimes and Raman finger prints. In this study the optical properties of normal hemoglobin are compared with glycated hemoglobin under non‐denaturing conditions in order to evaluate their potential as diagnostic and/or investigative biomarkers for chronic hyperglycemic conditions such as Diabetes mellitus Type 1 and 2. Support or Funding Information NxGen Partners, Ross Tower, 500 N. Akard Street, Suite 1820 Dallas TX 75201