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Role of Primary Cilia in Cell Migration
Author(s) -
Ganesh Jyothsna,
Miskevich Frank
Publication year - 2017
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.31.1_supplement.lb164
Subject(s) - microbiology and biotechnology , motility , cilium , cell migration , matrigel , biology , wound healing , cell , immunology , biochemistry
Cellular migration is a common activity for many different types of cells. If cellular migration is disrupted, cells may be unable to repair damaged tissue (wound repair), migrate appropriately as in cancer, or correctly signal their cellular state. In several situations, cilia have been implicated in helping to determine the direction of cellular migration. As primary cilia express a number of distinct receptors for particular hormones, these organelles have the required machinery to influence cellular behavior. We are using ARPE‐19 cells, a human retinal pigment epithelial cell line capable of forming cilia in culture, as an assay for cellular motility. Using a 3D model of cellular migration (matrigel invasion chambers) and a 2D scratch assay for cellular motility, we are testing various reagents for their ability to alter RPE cell motility. Preliminary results suggest that a number of agents which alter ciliary function, particularly protein kinase A modulators, are able to inhibit the migratory ability of ARPE cells. These results suggest that protein kinase A may be a key link between ciliary signaling and the direction of cellular migration. Support or Funding Information University of Michigan Graduate Student Research Assistantship; University Research and Creative Activities grant