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Paspalum scrobiculatum polyphenol rich extracts inhibit human cervical cancer cell proliferation and induce apoptosis in vitro
Author(s) -
Ramasamy Sakthivel,
Anupam K,
Ratnavathi C.V,
Karunagaran D,
Chandra T.S
Publication year - 2017
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.31.1_supplement.lb162
Subject(s) - hela , apoptosis , polyphenol , paspalum , chemistry , cell growth , cinnamic acid , reactive oxygen species , biochemistry , in vitro , antioxidant , biology , botany
Natural products and their derivatives have historically been a major source of new pharmaceuticals. Many dietary derivatives act as an effective anticancer agents such as curcumin, resveratrol and tea polyphenols which selectively inhibits the growth of malignant cells by inducing apoptosis. Cereals are valuable source of bioactive compounds which are found to be very effective in preventing and treating several diseases. Paspalum scrobiculatum is a type of small seeded grain (Kodo Millet) mainly consumed by India and also in Indonesia, Philippines, Thailand, Vietnam and in Africa. It has found its use in traditional medicines against inflammatory diseases, diabetes and several other diseases. In the present study, polyphenol‐rich extract (PEKM) of kodo millet was evaluated for its ability to inhibit the growth of human cervical cancer line (HeLa), at concentrations ranging from 100–500μg/ml. With increasing concentration of PEKM, a decrease in cell proliferation was observed in HeLa cell lines. The concentrations needed for 50% growth inhibition (IC 50 ) were found to be 212μg/ml and 178μg/ml for Kodo Millet Market (KMM) and Kodo Millet Vamban (KMV), respectively. The growth inhibition by PEKM was accompanied by membrane blebbing, appearance of apoptotic bodies, loss of mitochondrial membrane potential, increased production of reactive oxygen species and DNA damage, typically seen during apoptosis. In addition, it activated caspase‐9, decreased the expression of Bcl‐2 and induced poly ADP‐ribosome polymerase (PARP) cleavage. The phytochemical profiling of PEKM by HPLC revealed the presence of phenolic acids like quercetin and cinnamic acid in it. These results suggest that PEKM possesses the ability to inhibit cell growth in a dose dependent manner and could stimulate apoptosis in cancer cells. Support or Funding Information Indian Council of Agricultural Research (ICAR) and Indian Institute of Millets Research (IIMR), Govt. of India, New Delhi ‐110001