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Using Metabolite Profiling to Understand the Consequences of Iron‐Source Choices by Staphylococcus aureus
Author(s) -
DuBois Jennifer,
Celis Arianna
Publication year - 2017
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.31.1_supplement.lb139
Subject(s) - staphylococcus aureus , heme , metabolite , hemoglobin , bacteria , metabolite profiling , lysis , metabolome , chemistry , microbiology and biotechnology , computational biology , biochemistry , biology , enzyme , genetics
Bacteria inhabiting different environments are presented with different sources of nutritional iron. Major metabolic decisions are often made based on what sources of iron are available, and how limited those sources might be. For Staphylococcus aureus, heme iron is preferred under pathogenic conditions, where the bacteria survive by lysing red blood cells and consuming hemoglobin. We therefore hypothesize that Staphylococcus aureus may regulate a whole variety of metabolic decisions based on the sensed availability of heme. To test this hypothesis and examine the fate of heme and non‐heme iron sources, we are using NMR‐ and mass spectrometric based methods to show how whole‐cell metabolite pools change in response to changes in iron sources. Results from initial studies will be presented. Support or Funding Information NIH R01 GM‐090260