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Perivascular Adipose Tissue Modulates the Anticontractile Effect of Cooling in the Rat Aorta
Author(s) -
Rafique Yasmeen,
AlBader Maie,
Oriowo Mabayoje
Publication year - 2017
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.31.1_supplement.999.4
Subject(s) - aorta , enos , medicine , adipose tissue , endocrinology , chemistry , nitric oxide , anatomy , cardiology , nitric oxide synthase
The perivascular adipose tissue (PVAT) provides mechanical support for blood vessels and exerts an anti‐contractile effect. The present study was designed to investigate how PVAT and gender affect the anti‐contractile effect of cooling in the rat aorta. Aorta segments, with or without PVAT from male and female SD rats were used in this investigation. PE (10 −9 M – 10 −5 M) induced concentration‐dependent contractions of aorta segments at 37°C and 24°C. The maximum response and pD 2 values were reduced by PVAT in both sexes at 37°C. The reduction in pD 2 value was significantly greater in aorta segments from female rats. Cooling (24 °C) reduced the maximum response but not pD 2 values to PE in aorta segments without PVAT in both sexes. The anti‐contractile effect of cooling was attenuated in the presence of PVAT. Apocynin (3×10 −4 M) equipotently inhibited PE‐induced contractions both temperatures. L‐NAME (10 −4 M) potentiated PE‐induced contractions more in aorta segments without PVAT at both temperatures. The expression of eNOS protein and basal level of nitric oxide (NO) were greater in aorta segments with PVAT at both temperatures. However, PE significantly increased levels of NO only in aorta segments without PVAT. We concluded that PVAT attenuated the anti‐contractile effect of cooling by reducing generation of NO. Support or Funding Information Supported by the College of Graduate Studies and a grant (MR 05/15) from Kuwait University Research Sector.