Premium
Modulation of opioid and serotonergic systems by alpha‐terpineol complexed in beta‐cyclodextrin reduces hyperalgesia in a chronic muscle pain model
Author(s) -
Guimarães Adriana Gibara,
Oliveira Makson Gleydson Brito,
Menezes Paula Passos,
Souza Araújo Adriano Antunes,
Silva Juliane Cabral,
Silva Almeida Jackson Roberto Guedes,
Santos Márcio Roberto Viana,
Júnior Lucindo José Quintans
Publication year - 2017
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.31.1_supplement.985.21
Subject(s) - hyperalgesia , chemistry , pharmacology , (+) naloxone , serotonergic , opioid , antagonist , opioid receptor , morphine , saline , receptor antagonist , μ opioid receptor , anesthesia , medicine , nociception , receptor , serotonin , biochemistry
The aim of this study was to evaluate the effect of α‐terpineol, an alcohol monoterpene found in the essential oil of aromatic species, complexed in β‐cyclodextrin (TP‐βCD) in chronic muscle pain noninflammatory model and its mechanism of action. The model of chronic muscle pain was induced by two injections of pH 4.0 saline (20 μl) into the left gastrocnemius 5 days apart. After confirming hyperalgesia, male mice were treated with α‐terpineol (αTPN 100 mg/kg, p.o.), TP‐βCD (25, 50 or 100 mg/kg; p.o.) or vehicle (saline 0.9%, p.o.) daily for 10 days. The possible involvement of the opioid and serotonergic systems were evaluated using formalin test (1%; 20 μL; i.pl.), which animals were pretreated with αTPN‐βCD (p.o.) or vehicle (p.o.), naloxone (an opioid antagonist, 5 mg/kg; i.p.) or ondansetron (an 5HT 3 antagonist, 0.5 mg/kg; i.p.). The experimental protocols were approved for the UFS Ethic Committee (CEPA: 08/11). TP‐βCD reduced significantively (p<0.001) the mechanical hyperalgesia when compared with vehicle. Besides, the complexation with βCD increased the effect time of TP of 3 hrs to 7 hrs. Naloxone and ondansetron were able to reverse this analgesic effect. These results demonstrate that TP‐βCD reduced the hyperalgesia followed by the chronic muscle pain model, probably evoked by the descending inhibitory pain system, specifically by opioid and serotoninergic receptors. Support or Funding Information FAPITEC/SE, CAPES, CNPq (Brazil).