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An Integrated Study of Gene Expression Profile Uncovers Similarity between Embryogenesis, Bone Development, Wound Healing, and Prostate Cancer
Author(s) -
Rayburn Tyeler S,
Mukherjee Angana,
Byrd William A,
Jones Jacqueline
Publication year - 2017
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.31.1_supplement.980.2
Subject(s) - prostate cancer , foxa1 , biology , transcription factor , prostate , gene expression profiling , gene expression , gene , cancer research , cancer , bioinformatics , genetics
Embryogenesis, bone development, wound healing and cancer are intersecting processes that share common metabolic processes, growth factors and transcription factors. While solid tumors are controllable, bone metastases remain a death sentence, which is due to the complex area of the bone. Interestingly, studies have shown that 100% of prostate cancer deaths involve bone metastases. Although the role of transcription factors in embryogenesis, bone development, wound healing, and prostate cancer are studied separately, the overlapping of factors or genes across the processes remains unexplored. This study aims to develop an approach for identifying the overlapping gene signatures and analyzing their expression and correlation with embryogenesis, bone development, wound healing, and prostate cancer. We extracted data for the 4 processes from Gene Expression Omnibus (GEO); accession numbers: GSE18290 (n=9), GSE51812 (n=12), GSE24742 (n=12) and GSE3325 (n=19). Twenty overlapping genes were randomly identified and statistical analysis performed to compare the gene expressions across all the processes. Most genes in a cluster analysis exhibited common expression patterns in all the processes. Interestingly, a PCA analysis revealed a clear positive correlation between prostate cancer and bone development. Furthermore RUNX2, FGFR1, TWIST1, FOXP1, FOXA1, NKFB1, ELF1, PDGFA, IL6, JAG1, JAG2, and PDGFA were significantly up‐regulated in prostate cancer. Thus, we concluded that the transcription factors that play a role in the fundamental processes of early bone development, embryogenesis, and wound healing also play a critical role in prostate cancer bone metastases. We have identified, using a novel gene expression profiling together with a meta‐analysis validation approach, a set of overlapping gene signatures in embryogenesis, bone development, wound healing and prostate cancer, which can be used as prognostic markers for prostate cancer bone metastasis. Support or Funding Information This work was supported by the Department of Environmental and Biological Sciences, Troy University.