Premium
Left ventricular diastolic dysfunction in a model of obesity induced by a high carbohydrate diet
Author(s) -
Alves Pedro Henrique Rizzi,
Hasimoto Fabiana,
Kitawara Koody,
Fransciqueti Fabiane,
Ferron Artur,
Bazan Silméia,
Campos Dijon,
Ferreira Ana Lúcia,
Corrêa Camila
Publication year - 2017
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.31.1_supplement.976.7
Subject(s) - isovolumetric contraction , diastole , medicine , interventricular septum , ventricle , cardiology , diastolic function , blood pressure
Aim Evaluate the effect of diet obesity‐induced rich CHO on left ventricular diastolic function. Methods 16 male Wistar rats were assigned to receive water plus standard diet (group C, n = 8) and Ob diet with water plus 25% sucrose (Ob group, n = 8). Weekly body weight (BW) of the animals was assessed and, after 30 weeks of experiment, adiposity índex (AI). The following cardiac structures were measured: diastolic diameter (LVDD) and systolic (LVSD) LV; diastolic and systolic thickness posterior wall of the left ventricle (LVPWD; LVPWS) and interventricular septum (LVISD; LVISS); diameter of the aorta (DA), left atrium (LA) and the ratio (DA/LA); The relative thickness of the LV (ERVE); LV mass (LVM); MVE index (LVMI) was calculated by normalizing to body weight estimated LV mass. The LV systolic function was assessed by percentage of endocardial shortening (Δ% endo); The LV diastolic function was evaluated by the following indices: peak velocity of early diastolic filling (E wave); peak velocity of late diastolic filling (A wave); ratio between the E and A waves (E/A); deceleration time of E wave (DTE); isovolumetric relaxation time in absolute values (IRT) and normalized for heart rate (IRTn = IRT/R‐R 0,5). The study was supplemented by evaluation by tissue Doppler early diastolic (E′) and late (A′) of the mitral annulus (arithmetic average travel speeds of lateral and septal walls), and the ratio the waves (E/E′ and E′/A′). Results The ob group showed significantly higher weight (C = 493,008 ± 50,775, Ob = 562,95 ± 53,69; p < 0,05) and also higher adiposity index (C = 4.79 ± 0.73, Ob = 8.68 ± 1.76; p < 0,05). Results of the cardiac evaluation at 30 weeks are described in Table 1. Conclusion Consumption of carbohydrate‐rich diets promotes left ventricular diastolic dysfunction associated with obesity at 30 weeks. 1 Results of the cardiac evaluation performed at the end of 30 weeks.GRUPSVariables C Ob PBW (g) 493.008 ± 50.775 562.95 ± 53.69 0.018LVDD (mm) 7.206 ± 0.201 6.705 ± 0.560 0.031LVSD (mm) 3.116 ± 0.190 3.163 ± 0.431 0.785LVPWD (mm) 1.626 ± 0.190 1.723 ± 0.226 0.373LVPWS (mm) 3.039 ± 0.314 3.355 ± 0.213 0.033LVISD(mm) 1.659 ± 0.194 1.864 ± 0.308 0.133LVISS (mm) 3.355 ± 0.213 3.578 ± 0.193 0.045DA (mm) 3.736 ± 0.129 3.993 ± 0.135 0.001LA (mm) 4.756 ± 0.129 5.173 ± 0.383 0.011LA/DA 1.274 ± 0.059 1.297 ± 0.107 0.609LVDD/BW 14.755 ± 1.604 11.989 ± 1.304 0.002LA/BW 9.740 ± 1.087 9.243 ± 0.897 0.335LVM 0.817 ± 0.150 0.823 ± 0.152 0.937LVMI 1.669 ± 0.318 1.475 ± 0.312 0.240ERVE 0.451 ± 0.050 0.519 ± 0.095 0.098CF (bpm) 234.875 ± 39.364 295.500 ± 26.966 0.002Cardiac Output 81.181 ± 18.120 81.834 ± 28.74 0.957% endo 56.743 ± 2.615 52.397 ± 8.591 0.190E wave 68.350 ± 5.258 73.138 ± 3.312 0.046A wave 40.675 ± 3.656 45.725 ± 6.040 0.062E/A 1.684 ± 0.088 1.617 ± 0.163 0.327IRT‐ms 21.125 ± 2.232 23.250 ± 3.012 0.131DTE‐ms 47.250 ± 3.059 50.625 ± 2.722 0.035R‐R (seg) 0.261 ± 0.042 0.205 ± 0.019 0.003IRT/R‐R 41.696 ± 5.773 51.420 ± 5.826 0.004Ejection fraction 0.918 ± 0.015 0.883 ± 0.063 0.142E′ lateral 5.588 ± 0.412 5.225 ± 0.776 0.262A′ lateral 3.550 ± 0.493 4.050 ± 0.578 0.083E′ septal 5.850 ± 0.404 5.788 ± 0.906 0.861A′ septal 3.950 ± 0.946 4.863 ± 0.691 0.044E′ 5.791 ± 0.383 5.506 ± 0.735 0.479A′ 3.750 ± 0.410 4.456 ± 0.540 0.010E/E′ 12.016 ± 1.397 13.492 ± 1.867 0.093E′/A′ 1.535 ± 0.145 1.260 ± 0.253 0.018