Premium
Histopathological dissection of three different osteoporosis animal models including ovariectomy female, F VIII knockout and Akr1A1 knockout mice
Author(s) -
Lin WeiYu,
Chang RoLin,
Liu YaoWen,
Chen ChuanMu
Publication year - 2017
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.31.1_supplement.976.4
Subject(s) - knockout mouse , osteoporosis , endocrinology , osteocalcin , medicine , bone mineral , bone density , alkaline phosphatase , chemistry , receptor , biochemistry , enzyme
Osteoporosis is defined as a skeletal disorder characterized by compromised bone strength, predisposing individuals to an increased risk of fracture. In our previous studies, we established two different osteoporosis animal models including coagulation F VIII knockout mice (genetic defect model) and Akr1A1 knockout mice (nutritional imbalanced model). We found that the degrees of bone loss in these two models are different from traditional ovariectomy (OVX) model, therefore we use micro‐computed tomography (μ‐CT) to analysis bone microarchitecture changes in these three different osteoporosis models. Six groups were designed for bone histopathological analyses in this study, including male and female B6 mice, OVX B6 mice, male ICR mice, male F VIII knockout mice and male Akr1A1 knock‐out mice. In the analysis of femurs trabecular bone parameters, bone volume/tissue volume (BV/TV) and trabecular separation (Tb.Sp) were most serious loss in OVX group; whereas trabecular thickness (Tb.Th), trabecular number (Tb.N) and trabecular bone mineral density (Tb.BMD) were most serious loss in F VIII knockout group. It is worth mentioning that although Akr1A1 knockout mice exhibited bone loss compared with control (ICR) group, but not significantly loss than F VIII knockout mice and OVX mice. In the image of femurs cortical bone, bone morphology was obviously shorter in length enlarged in cavity in Akr1A1 knockout mice. In the blood biochemical parameters, carboxyl‐terminal cross‐linking telopeptides of type I collagen (CTX‐1) was highest in Akr1A1 knockout mice, alkaline phosphatase (ALP) and osteocalcin (OC) were significantly decreased in OVX group. Present data demonstrate that the animal models of OVX, F VIII knockout and Akr1A1 knock‐out can be used to represent different types of osteoporosis and to provide feasible platforms for the investigations of osteoporosis and drug development.